
Background: D-Luciferin is a firefly luciferase substrate with a quantum 0.88, which is 20 times that of Luminol. Reaction principle: First, luciferase reacts fluorescein presence of magnesium ions, which are then oxidized structure and emit yellow-green light.

Application: 1.Imaging analysis of in vivo/in vitro expression of LUC marker gene and luciferase-fusion gene in living cells, tissues or organisms. 2.It is widely used for report gene analysis, immunoassay and ATP fluorescence health monitoring analysis.

4) After injection into the body for 10-15 minutes (when the reaches the strongest stable platform period), imaging analysis out. Note: it is suggested that luciferase kinetic curve should be each animal model to determine the maximum signal detection